Gel Electrophoresis

 

1. Using the Invitrogen E-Gel system, run a 2% single or double comb gel on the PCR products to confirm and quantify amplicon concentration. Be sure to pre-run your gel by running the blank gel for 2min at 60V

2. In each well load in 10μL of DI H₂0 along with 5μL of sample for the double combed gel.   For the single combed gel, load 15μL DI H₂O along with 5μL of your sample. Add 10μL of the DNA ladder in the well designated for the ladder. For unused wells, load with 10μL of DI H₂O

3. Run the single combed gels at 60V for approximately 25min.  For the double combed gels, run for approximately 17min at 60V.

4. Once completed, visualize and quantify concentration of amplicons with the BioRad gel dock system.